Profiling grapevine trunk pathogens in planta: a case for community-targeted DNA metabarcoding
نویسندگان
چکیده
منابع مشابه
fungal pathogens associated with grapevine trunk diseases in iran
during 2004–2007 various own rooted vineyards were inspected to study the fungi associated with vine trunk diseases in iran. samples from declining vines showing yellowing and reduced growth and different symptoms in wood, including browning of the wood, necrosis, brown and black streaking and white rot were collected. fungal isolations were made from affected tissues onto malt extract agar (me...
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Grapevines, like other perennial crops, are affected by so-called 'trunk diseases', which damage the trunk and other woody tissues. Mature grapevines typically contract more than one trunk disease and often multiple grapevine trunk pathogens (GTPs) are recovered from infected tissues. The co-existence of different GTP species in complex and dynamic microbial communities complicates the study of...
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A significant reduction in vine production has recently been recorded in several vine regions in Algeria due to the death (total or partial) of many vines and to the pulling of many vineyards that had showed too many symptoms of decline. This study was conducted during spring 2010 2012 to detect the responsible agents for this disease. Samples of grapevine wood were collected in five grapevine ...
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Next generation metabarcoding is becoming an indispensable tool in fungal community ecology. Here we tested Illumina metabarcoding, a method that generates shorter reads but achieves deeper sequencing than 454 metabarcoding approaches. We found that paired-end Illumina MiSeq data cover the full ITS1 in many fungal lineages and are suitable for environmental fungal community assessment. There wa...
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ژورنال
عنوان ژورنال: BMC Microbiology
سال: 2018
ISSN: 1471-2180
DOI: 10.1186/s12866-018-1343-0